IL-23 costimulates antigen-specific MAIT cell activation and enables vaccination against bacterial infection

Huimeng Wang; Lars Kjer-Nielsen; Mai Shi; Criselle D'Souza; Troi J Pediongco; Hanwei Cao; Lyudmila Kostenko; Xin Yi Lim; Sidonia BG Eckle; Bronwyn S Meehan; Tianyuan Zhu; Bingjie Wang; Zhe Zhao; Jeffrey YW Mak; David P Fairlie; Michele WL Teng; Jamie Rossjohn; Di Yu; Barbara Fazekas de St Groth; George Lovrecz; Louis Lu; James McCluskey; Richard A Strugnell; Alexandra J Corbett; Zhenjun Chen

Journal article

IL-23 costimulates antigen-specific MAIT cell activation and enables vaccination against bacterial infection

Huimeng Wang, Lars Kjer-Nielsen, Mai Shi, Criselle D'Souza, Troi J Pediongco, Hanwei Cao, Lyudmila Kostenko, Xin Yi Lim, Sidonia BG Eckle, Bronwyn S Meehan, Tianyuan Zhu, Bingjie Wang, Zhe Zhao, Jeffrey YW Mak, David P Fairlie, Michele WL Teng, Jamie Rossjohn, Di Yu, Barbara Fazekas de St Groth, George Lovrecz Show all

SCIENCE IMMUNOLOGY | AMER ASSOC ADVANCEMENT SCIENCE | Published : 2019

DOI: 10.1126/sciimmunol.aaw0402

Download PDF

Abstract

Mucosal-associated invariant T (MAIT) cells are activated in a TCR-dependent manner by antigens derived from the riboflavin synthesis pathway, including 5-(2-oxopropylideneamino)-6-D-ribitylaminouracil (5-OP-RU), bound to MHC-related protein-1 (MR1). However, MAIT cell activation in vivo has not been studied in detail. Here, we have found and characterized additional molecular signals required for optimal activation and expansion of MAIT cells after pulmonary Legionella or Salmonella infection in mice. We show that either bone marrow–derived APCs or non–bone marrow–derived cells can activate MAIT cells in vivo, depending on the pathogen. Optimal MAIT cell activation in vivo requires signalin..

View full abstract

University of Melbourne Researchers

Huimeng Wang Author

Sidonia Eckle Author

Bronwyn Meehan Author

Jamie Rossjohn Author

Related Projects (6)

ANTIGEN PRESENTATION, RECOGNITION AND THE IMMUNE RESPONSE

This program focuses on understanding the development of immunity during infection or inflammatory diseases using a broad array of technique..

MAIT CELLS IN BACTERIAL INFECTION. FRIEND OR FOE?

A specialised set of T lymphocytes called Mucosal Associated Invariant T (MAIT) cells react against bacteria and yeast, and reside at mucosa..

Understanding recognition of vitamin B-based antigens by the immune system

This project aims to evaluate the range of molecules that can stimulate vitamin B-reactive T cells in mammals and amphibians, and the degree..

Cell facilitated controlled radical polymerization

This project aims to develop a controlled polymerisation method by combining reversible addition fragmentation chain (RAFT) polymerisation t..

A STRUCTURAL INVESTIGATION INTO T CELL SIGNALLING MACHINES

The project aims to understand how receptor recognition events cause intracellular signalling.Membrane-bound receptors, their cognate ligand..

NHMRC PROGRAM IN CELLULAR MICROBIOLOGY

Infectious diseases plague mankind; with infections responsible for approximately 20% of all deaths worldwide. New strategies are urgently n..

Grants

Awarded by National Health and Medical Research Council of Australia

Awarded by Australian Research Council

Awarded by ARC Future Fellowship

Awarded by NHMRC Senior Principal Research Fellowship

Awarded by ARC Australian Laureate Fellowship

Funding Acknowledgements

We acknowledge the support of the National Health and Medical Research Council of Australia (program grants 1113293 and 1092262 and project grant 1120467) and the Australian Research Council (Discovery Project DP170104321). A.J.C. was supported by an ARC Future Fellowship FT160100083. D.P.F. was supported by an NHMRC Senior Principal Research Fellowship 1117017. J.R. was supported by an ARC Australian Laureate Fellowship FT160100049. H.W. was supported by a Melbourne International Engagement Award (The University of Melbourne). C.D. was supported by a Melbourne International Research Scholarship and a Melbourne International Fee Remission Scholarship (The University of Melbourne).